Journal: Blood Advances

Article Title: Mesothelin is a novel cell surface disease marker and potential therapeutic target in acute myeloid leukemia

doi: 10.1182/bloodadvances.2021004424

Figure Lengend Snippet: In vitro and in vivo cytotoxicity of MSLN-targeted ADCs in MSLN+leukemia cell lines. (A) In vitro cytotoxicity of AR in MV4;11-MSLN+ cell lines and IC50 values. Controls are IC-AR and treatment of the parental (MSLN−) lines. In vitro cytotoxicity of the ADC anti-MSLN–DGN462 with an indolino-benzodiazepine dimer payload in MV4;11-MSLN+ and MV4;11 parental cells (B) and Nomo-1 parental cells and Nomo-1–MSLNKO cells (C). (D) Kaplan-Meier survival plots of K562-MSLN+ cell–xenografted mice treated with AR compared with IC-AR, chemotherapy (Chemo), and no treatment. (E) Kaplan-Meier survival plots of MV4;11-MSLN+ xenografted mice treated with AR, along with controls: IC-AR (IC) and untreated. (F) PB leukemia burden was assessed in MV4;11-MSLN+ mice by flow cytometry. (G) Treatment of MSLN+ PDX NTPL-146 with AR resulted in a dose-dependent improvement in median survival with respect to untreated mice. Mice treated with AR vs IC-AR for 2 cycles (dashed lines; n = 5 per group) experienced a median survival of 82 days vs 32 days (P = .0018) and mice treated for 3 cycles (solid lines; n = 4 per group) had a median survival of 132 days vs 33 days, respectively (P = .0069; n = 4 per group). (H) Treatment of the MSLN− PDX DF-2 with AR did not demonstrate any target-dependent efficacy compared with untreated IC-AR mice. Mice treated with AR vs IC-AR for 1 cycle (dotted lines; n = 4 per group) experienced an identical median survival of 4 days (P = 1.0) and mice treated for 2 cycles (solid lines; n = 5 per group) had identical median survival of 12 days, respectively (P = .173; n = 4 per group). (I) Quantification of cell surface mesothelin expression using BD Quantibrite, as measured by antibodies bound per cell in the MSLN+ ovarian cancer cell line OCVAR-3 used as positive control and the PDX models NTPL-146 and DF2. ND, IC50 could not be determined with 95% CIs.

Article Snippet: MSLN + and MSLN − cell lines Four MSLN − leukemia cell lines, K562 and Kasumi-1 (American Type Culture Collection) and Me-1 and MV4;11 (DSMZ), as well as 1 MSLN + AML cell line, Nomo-1 (DSMZ), were purchased and used for MSLN expression and cytotoxicity assays.

Techniques: In Vitro, In Vivo, Flow Cytometry, Expressing, Positive Control

Journal: Medical Physics

Article Title: Modification and validation of an analytical source model for external beam radiotherapy Monte Carlo dose calculations

doi: 10.1118/1.4955434

Figure Lengend Snippet: Calculated and measured percent depth dose curves for 6 MV, 4 × 4 cm field and 10 MV, 40 × 40 cm field.

Article Snippet: In Table , the 6 MV PDD data from Fig. are compared showing the local percent difference between the depths of 0.8 and 6.0 cm. fig ft0 fig mode=article f1 FIG. 5. caption a4 Calculated and measured percent depth dose curves for 6 MV, 4 × 4 cm field and 10 MV, 40 × 40 cm field. table ft1 table-wrap mode=article t1 TABLE I. caption a4 PDD comparison of the Varian 10 MV 40 × 40 cm field and the Varian 6 MV 4 × 4 cm field for a portion of the PDD from 0.8 to 6.0 cm depth showing the local percent difference.

Techniques: